A. FOR SCIENTISTS

1. Molecular cloning service:

    Assist investigators with plasmid manipulations and cloning projects

    The services include:

1. Endotoxin-free Plasmid purification, insert purification, plasmid management.
2. Other projects are designed upon the specific needs of client including custom subcloning, library screening etc.

    Assist investigators with protein expression and purification:

    The services include:

1. Generation of protein expression vectors.
2. Recombinant protein expression in bacterial cells (multiple systems).

    Assist investigators with comprehensive gene expression analysis:

    The services include:

1. RNA isolation from tissue or mammalian cell lines.
2. Real time- RT PCR analysis looking for differential gene expression at RNA level.
3. Immunological blotting looking for differential gene expression at protein level.

    Assist investigators with tissue culturing and maintaining cell line:

    The services include:

1. Generation of various human primary cell line.
2. Culturing and maintaining various mammalian cell lines.